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Objective:To explore the influencing factors of acute bilirubin encephalopathy (ABE) in neonates with severe hyperbilirubinemia.Methods:A total of 123 cases of severe neonatal hyperbilirubinemia (serum total bilirubin > 342 μmol/L) in our hospital from January 2018 to May 2020 were retrospectively analyzed.According to the occurrence of ABE, they were divided into ABE group (28 cases) and non-ABE group (95 cases). The perinatal data and laboratory examination results between two groups were compared.The variables with statistical differences in univariate analysis were included in multivariate Logistic regression analysis.Results:Univariate analysis showed that the hemoglobin level and hematocrit of ABE group were higher than those of non-ABE group.The total bilirubin value, length of hospital stay, natural childbirth, mixed feeding, infection with craniocerebral hemorrhage were all higher than those in the non-ABE group, and the differences were statistically significant( P<0.05). Multivariate Logistic regression analysis showed that high hemoglobin level ( OR=1.032, 95% CI 1.007 to 1.057) and long hospital stay ( OR=1.15, 95% CI 1.007 to 1.312) were independent risk factors for ABE patients.Breastfeeding was a protective factor for ABE patients( OR=0.151, 95% CI 0.028 to 0.821). Conclusion:High hemoglobin value and long hospital stay are independent risk factors for ABE patients, and breast feeding is a protective factor for ABE.
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Henoch-Schonlein purpura (HSP) is one of the most common clinical manifestations of systemic small vasculitis in children with non-thrombocytopenic, and mainly manifested as skin purpura, arthritis, gastrointestinal symptoms and Henoch-Schonlein purpura nephritis (HSPN). The severity of renal involvement is the main factor determining the long-term prognosis of children with HSP.Studies have revealed that the determination of pentraxin 3 (PTX3) in serum can be used for early diagnosis of HSPN and prediction of renal injury.In this paper, the origin, gene and protein structure, function, potential relationship and mechanism of action between PTX3 and HSP were discussed, so as to provide new ideas for the early diagnosis and treatment of HSPN.
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Objective@#To investigate the clinical relevant factors associated with transfusion associated necrotizing enterocolitis (TANEC) in order to reduce the incidence of neonatal necrotizing enterocolitis (NEC).@*Methods@#The clinical data of neonates admitted to the First Hospital of Lanzhou University and received blood transfusion therapy from January 2017 to June 2018 were collected, including perinatal factors, basic conditions of children, and comorbidities.According to the occurrence or absence of NEC within 48 hours after transfusion, the patients were divided into TANEC group and no-TANEC group.The clinical data of the two groups were compared.@*Results@#Univariate analysis showed that there were statistical differences (P<0.05) in the mode of delivery, gestational age, birth weight, neonatal sepsis, patent ductus arteriosus(PDA), neonatal respiratory distress syndrome (NRDS), and anemia.The logistic multivariate analysis revealed that gestational age(P<0.05, OR=0.772, 95%CI: 0.684-0.871), the birth weight(P<0.05, OR=0.236, 95%CI: 0.079-0.711)were protect fators of TANEC, the degree of anemia(mode 1: P<0.05, OR=3.129, 95%CI: 1.003-9.756; mode 2: P<0.05, OR=3.449, 95%CI: 1.024-11.609)and sepsis (mode 1: P<0.05, OR=6.327, 95%CI: 1.732-23.720; mode 2: P<0.05, OR=8.154, 95%CI: 2.122-31.336)were significant risk fators for TANEC.@*Conclusion@#The factors leading to the occurrence of TANEC are various.When transfused, the greater the gestational age, the higher the birth weight, the lower the risk of developing NEC.The more severe the combination of neonatal sepsis and anemia, the higher the risk of developing TANEC.Clinically, comprehensive measures should be taken to prevent neonatal anemia.According to the specific conditions of the children and the degree of anemia, a reasonable clinical strategy should be formulated to avoid blood transfusion to reduce the incidence of TANEC and improve the prognosis of the children.
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Objective:To evaluate the characteristics and risk factors for blood transfusion in very low birth weight infants(VLBWI).Methods:Clinical data of one hundred VLBWI, hospitalized from July, 2016 to June, 2019, were studied retrospectively.The infants were divided into two groups according to whether they received blood transfusion.The general information, incidence of diseases and treatment measures were compared between two groups.The risk factors influencing the blood transfusion were analyzed.Results:Of the one hundred VLBWI, sixty-nine cases needed blood transfusion.The first time of blood transfusion ranged from one to four weeks after birth, and average number of transfusions was 6 times.Maternal anemia during pregnancy, birth weight, gestational age, hemoglobin and hematocrit at birth, volume of blood taking within two weeks after birth, duration of hospitalization, duration of paraenteral nutrition, delivery method, need for intubation and neonatal respiratory distress syndrome, apnea, bronchopulmonary dysplasia, patent ductus arteriosus showed significant differences between the two groups( P<0.05). Logistic regression analysis revealed that lower gestation( OR=0.386, 95% CI 0.212-0.704, P=0.002), longer duration of hospital stay( OR=2.177, 95% CI 1.170-4.049, P=0.014), prolonged parenteral nutrition( OR=1.195, 95% CI 1.083-1.319, P<0.001), greater volume of blood taking within two weeks after birth ( OR=1.269, 95% CI 1.083-1.487, P=0.003)and cesarean delivery( OR=5.513, 95% CI 1.056-28.770, P=0.043) were associated with increasing risk of blood transfusion in VLBWI. Conclusion:The gestational age, length of hospital stay, blood intake within two weeks after birth, duration of paraenteral nutrition and delivery method all affected the risk of blood transfusion to varying degrees.
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Objective:To investigate the clinical relevant factors associated with transfusion associated necrotizing enterocolitis (TANEC) in order to reduce the incidence of neonatal necrotizing enterocolitis (NEC).Methods:The clinical data of neonates admitted to the First Hospital of Lanzhou University and received blood transfusion therapy from January 2017 to June 2018 were collected, including perinatal factors, basic conditions of children, and comorbidities.According to the occurrence or absence of NEC within 48 hours after transfusion, the patients were divided into TANEC group and no-TANEC group.The clinical data of the two groups were compared.Results:Univariate analysis showed that there were statistical differences ( P<0.05) in the mode of delivery, gestational age, birth weight, neonatal sepsis, patent ductus arteriosus(PDA), neonatal respiratory distress syndrome (NRDS), and anemia.The logistic multivariate analysis revealed that gestational age( P<0.05, OR=0.772, 95% CI: 0.684-0.871), the birth weight( P<0.05, OR=0.236, 95% CI: 0.079-0.711)were protect fators of TANEC, the degree of anemia(mode 1: P<0.05, OR=3.129, 95% CI: 1.003-9.756; mode 2: P<0.05, OR=3.449, 95% CI: 1.024-11.609)and sepsis (mode 1: P<0.05, OR=6.327, 95% CI: 1.732-23.720; mode 2: P<0.05, OR=8.154, 95% CI: 2.122-31.336)were significant risk fators for TANEC. Conclusion:The factors leading to the occurrence of TANEC are various.When transfused, the greater the gestational age, the higher the birth weight, the lower the risk of developing NEC.The more severe the combination of neonatal sepsis and anemia, the higher the risk of developing TANEC.Clinically, comprehensive measures should be taken to prevent neonatal anemia.According to the specific conditions of the children and the degree of anemia, a reasonable clinical strategy should be formulated to avoid blood transfusion to reduce the incidence of TANEC and improve the prognosis of the children.
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Henoch-Schonlein purpura (HSP) is a type of systemic leucocytoclastic vasculitis mediated by IgA immune complex deposition, which mainly affects the skin, joints, gastrointestinal tract and small blood vessels in the glomeruli.It is a common systemic vasculitis in children.Due to severe complications of HSP, conventional clinical medicine has limited efficacy in some serious, even life-threatening or organ dysfunction cases, such as advanced renal insufficiency, fatal gastrointestinal bleeding, nervous vasculitis, pulmonal bleeding, etc.As an adjunctive therapy, plasma exchange has led to significant results for this type of HSP.In this paper, the status of plasma exchange in HSP treatment was reviewed.
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Objective@#To analyze the genotypes, amino acid vatiations and molecular epidemiological characteristics of respiratory syncytial virus (RSV) infection in pediatric patients in Gansu province for the future research.@*Methods@#A total of 4 556 respiratory tract specimens were colleted from pediatric patients under 10 years of age in five cities in Gansu from 2012 to 2017. These specimens were tested for RSV and its subtypes.The coding region of the RSV G gene was amplified using reverse transcription-polymerase chain reaction (RT-PCR) and sequenced for RSV positive specimens. Sequences were edited using DNA Star software. Sequence alignment and phylogenetic trees were built by MEGA 6.0 software.@*Results@#Out of 4 556 specimens, 1 135 (24.91%) were positive for RSV, totally 216 G protein sequences were obtained. RSV A isolates were clustered into three genotypes: NA1、NA3 and ON1. The nucleotides and amino-acid homology was 84.9%-100% and 77.3%-100%, respectively. The nucleotides and amino-acid homology between this study and prototype long strain was 81.2%-83.3% and 74.1%-88.0%. RSV B isolates were clustered into only BA9 one genotypes. The nucleotides and amino-acid homology was 97.7%-100% and 95.8%-100%, respectively. The nucleotides and amino-acid homology between this study and prototype CH18537 strain was 84.9%-85.7% and 77.9%-80.1%.@*Conclusions@#The genetic characteristics and the amino-acid changes were analyzed systematically using data of RSV G gene collected from 2012 to 2017 in Gansu province in this study. These data were used for analyses of the etiology, control and prevention of RSV infection.
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Objective@#To analyze the infection status and pathogenic characteristics of rotavirus in group A of children under 5 years of age in Lanzhou city in 2017, and provide scientific data for prevention and control of rotavirus infection.@*Methods@#A total of 219 stool samples from children with diarrhea under the age of 5 years in the Department of Pediatrics, First Hospital of Lanzhou University from January to December in 2017 were collected. A group of human rotavirus positive samples were detected by ELISA, and G/P typing was detected by using reverse transcription-polymerase chain reaction (RT-PCR)) combined with sequencing. Some of the VP7 gene fragments were amplified, sequenced and analyzed for phylogenetic characteristics.@*Results@#A total of 113 rotavirus in group A positive samples were detected, with a total positive rate of 51.60%. The main target of infection was children 0 to 17 months, and the peak of the epidemic was mainly in November. G/P genotyping was performed: G9 was predominant G genotype (90.27%), followed by G2 (7.08%). P[8] was predominant P genotype (91.15%), followed by P [4] (7.96%). In 2017, the group A human rotavirus epidemic strain was mainly G9P[8](88.50%). Sequence analysis and phylogenetic analysis of VP7 gene fragments showed that the dominant genotypes G9 had higher homology with isotype reference strains in other regions of China.@*Conclusions@#Children 0 to 17 months old in Lanzhou city are at high risk of group A human rotavirus infection. G9P[8] is a dominant genotype.
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Objective:To investigate the therapeutic effect of folic acid combined with vitamin B12 on atherosclerosis induced by hyperhomocysteinemia in the rats,and to elucidate its therapeutic mechanism.Methods:Thirty-three rats were randomly divided into control group,model group and treatment group (n=11).The rats in control group were given normal diet,the rats in model group were given normal diet and methionine,and the rats in treatment group were given normal diet,methionine,perfused folic acid and vitamin B12.The histopathological changes of thoracic aorta of the rats were observed by HE staining.The levels of serum Hcy and matrix metalloproteinase-9 (MMP-9) were detected with ELISA,and the expression levels of CCR5 and CC chemokine ligand 5 (CCL5) proteins in thoracic aortic smooth muscle cells were detected by immunohistochemical staining.The expression levels of CCR5 and CCL5 mRNA in thoracic aortic smooth muscle cells were detected by RT-PCR.Results:The results of HE staining showed that the medial smooth muscle cells of the rat thoracic aorta were arranged neatly in control group,the intimal elastic membrane was complete and the endothelial cells were continuous;the smooth muscle cells of the thoracic aorta were loose in model group,the endometrium was damaged,the foam cells were formed under the intima,and the endothelial cells were necrotic;the pathological changes of the thoracic aorta of the rats in treatment group were between control group and model group.The levels of serum Hcy of the rats in model group and treatment group were higher than that in control group (P＜0.05);the serum Hcy level in treatment group was lower than that in model group (P＜0.05).The levels of serum MMP-9 of the rats in model group and treatment group were higher than that in control group (P＜0.05);the serum MMP-9 level in treatment group was lower than that in model group (P＜0.05).The expression gray values of CCR5 and CCL5 proteins in the thoracic aortic smooth muscle cells of the rats in rats in model group and treatment group were lower than those in control group (P＜0.05);the expression gray values of CCR5 and CCL5 proteins in the thoracic aortic smooth muscle cells of the rats in treatment group were higher than those in model group (P＜0.05).The relative expression levels of CCR5 and CCL5 mRNA in the thoracic aortic smooth muscle cells of the rats in model group and treatment group were higher than those in control group (P＜0.05);the relative expression levels of CCR5 and CCL5 mRNA in the thoracic aortic smooth muscle cells of the rats in treatment group was lower than that in model group (P＜0.05).Conclusion:Folic acid combined with vitamin B12 could decrease the serum Hcy and MMP-9 levels and the expression levels of CCR5 and CCL5 in the thoracic aortic smooth muscle cells which may be one of the mechanisms of folic acid combined with vitamin B12 in the treatment of atherosclerosis.
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Objective:To investigate the therapeutic effect of folic acid combined with vitamin B12 on atherosclerosis induced by hyperhomocysteinemia in the rats,and to elucidate its therapeutic mechanism.Methods:Thirty-three rats were randomly divided into control group,model group and treatment group (n=11).The rats in control group were given normal diet,the rats in model group were given normal diet and methionine,and the rats in treatment group were given normal diet,methionine,perfused folic acid and vitamin B12.The histopathological changes of thoracic aorta of the rats were observed by HE staining.The levels of serum Hcy and matrix metalloproteinase-9 (MMP-9) were detected with ELISA,and the expression levels of CCR5 and CC chemokine ligand 5 (CCL5) proteins in thoracic aortic smooth muscle cells were detected by immunohistochemical staining.The expression levels of CCR5 and CCL5 mRNA in thoracic aortic smooth muscle cells were detected by RT-PCR.Results:The results of HE staining showed that the medial smooth muscle cells of the rat thoracic aorta were arranged neatly in control group,the intimal elastic membrane was complete and the endothelial cells were continuous;the smooth muscle cells of the thoracic aorta were loose in model group,the endometrium was damaged,the foam cells were formed under the intima,and the endothelial cells were necrotic;the pathological changes of the thoracic aorta of the rats in treatment group were between control group and model group.The levels of serum Hcy of the rats in model group and treatment group were higher than that in control group (P＜0.05);the serum Hcy level in treatment group was lower than that in model group (P＜0.05).The levels of serum MMP-9 of the rats in model group and treatment group were higher than that in control group (P＜0.05);the serum MMP-9 level in treatment group was lower than that in model group (P＜0.05).The expression gray values of CCR5 and CCL5 proteins in the thoracic aortic smooth muscle cells of the rats in rats in model group and treatment group were lower than those in control group (P＜0.05);the expression gray values of CCR5 and CCL5 proteins in the thoracic aortic smooth muscle cells of the rats in treatment group were higher than those in model group (P＜0.05).The relative expression levels of CCR5 and CCL5 mRNA in the thoracic aortic smooth muscle cells of the rats in model group and treatment group were higher than those in control group (P＜0.05);the relative expression levels of CCR5 and CCL5 mRNA in the thoracic aortic smooth muscle cells of the rats in treatment group was lower than that in model group (P＜0.05).Conclusion:Folic acid combined with vitamin B12 could decrease the serum Hcy and MMP-9 levels and the expression levels of CCR5 and CCL5 in the thoracic aortic smooth muscle cells which may be one of the mechanisms of folic acid combined with vitamin B12 in the treatment of atherosclerosis.
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We studied the epidemiological characteristics of human bocavirus 4 (HBoV4) in children with a- cute gastroenteritis in Lanzhou (China). A total of 331 stool specimens were collected from children aged < 5 years with acute diarrhea at the First Hospital of Lanzhou University between July 2012 and June 2013. Specimens of HBoV were identified by nested polymerase chain reaction assays. Compared with related sequences in GenBank, the HBoV-positive strain isolated in the present study was,quite surprisingly, a rare genotype named HBoV4. This strain was a typical HBoV4,with high levels of nucleotide and amino acid homology to the Thailand strain, JQ267789 (98.9% and 98.7%, respectively), and the USA strain, GQ506568 (97.6% and 97.4%, respectively). This is the first report of HBoV4 as the causative agent for acute gastroenteritis in pediatric patients in China. This strain is one of two genotypes of HBoV that are currently circulating.
Subject(s)
Child, Preschool , Female , Humans , Infant , Male , China , Feces , Virology , Gastroenteritis , Virology , Human bocavirus , Classification , Genetics , Molecular Sequence Data , Parvoviridae Infections , Virology , PhylogenyABSTRACT
MicroRNA (miRNA) are small non-coding molecules of ribonucleic acid. They are about 22 nucleotides in length, single-stranded, and mediate post-translational regulation by the repression or degradation of messenger RNA(mRNA). miRNA play a key part in the proliferation, differentiation and death of cells. Viral infection is one of the most common causes of human disease. Some studies have found that miRNA has a very close relationship with viral infection, which has an effect on viral replication, the immune response and antiviral immunity. Use of miRNA may become the cornerstone of new methods for the diagnosis and treatment of viral infection. This article summarizes the progress of research into miRNA and viral infection.
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Animals , Humans , MicroRNAs , Genetics , Metabolism , Virus Diseases , Genetics , Metabolism , Virology , Virus Physiological Phenomena , Virus Replication , Viruses , GeneticsABSTRACT
Enterovirus 71 (EV71) is a major agent of hand, foot and mouth disease that can cause a severe burden of disease to children. To identify an effective method for the control and prevention of EV71, we studied the effect of exposure to heat and ultraviolet (UV) light upon EV71 inactivation. We found that exposure to 50 degrees C could not inactivate the infectivity of EV71. However, exposure to 60 degrees C and 70 degrees C could inactivate EV71 effectively. EV71 could be inactivated after exposure to UV light at a distance between the sample and a lamp of 30 cm for 30 min or 60 min because viral genomic RNA was destroyed. However, fetal bovine serum (FBS) could attenuate the inactivation proffered by heat and UV light. Attenuation effects of FBS were correlated positively with FBS concentration. Hence, EV71 can be inactivated by exposure to heat and UV light, and our results could provide guidance on prevention of the spread of EV71.
Subject(s)
Humans , Disinfection , Methods , Enterovirus A, Human , Genetics , Physiology , Radiation Effects , Enterovirus Infections , Virology , Hot Temperature , Ultraviolet Rays , Virus Inactivation , Radiation EffectsABSTRACT
Objective To explore the role of mammalian target of rapamycin (mTOR) complex in the adriamycin induced the podecytes.Methods 1 μmol/L adriamycin (ADM) was used to induce mice podocytes for 24 hours and detected the apoptosis ratio,podosytes damage markers (Desmin) and the related expression of mTOR,then the role of mTOR was analysized in ADM induced podosytes.Results 1 umol/L ADM induced mice podosytes for 24 hours can make the podocytes damage.Compared with control group,podosytes activity declined(t =28.68,P ＜ 0.001),apoptosis rate increased both early (t =10.24,P =10.24) and late (t =4.26,P =4.26),and Desmin expression increased(t =6.16,P ＜0.001).mTOR expression decreased in ADM group.Compared with control group,both mTOR and phosphorylation mTOR expression decreased (t =3.57,P =3.57).mTOR downstream target molecular S6K1 and 4ebpl mRNA expression decreased(t =18.28,P ＜ 0.001).Conclusion ADM can make podosytes damage and the mechanism of ADM induced podosytes damage may be related to the decreasing mTOR and the low activity of mTOR.
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Objective To evaluate the effects and safety of tarcrolimus on children difficult nephritic syndrome.Methods Databases including the Cochrane Library,Pubmed,Medline,OVID,CNKI,Wan Fang Data and VIP were searched to collect the controlled trials on tacrolimus capsule published from Jan 2003 to Jun 2013.The quality of the included randomized controlled trials was assessed by Jadad,and the complete remission,the fail,the relapsing rate of 12 month and side effects after treatment were extracted,meta-analysis was performed using RevMan 5.0 software.Results Among 179 articles,6 articles were included,4 of them were English and the other 2 were Chinese.The results of meta-analysis based on stratified therapeutic strategies showed that:(1) comparing with cyclophosphamide,tacrolimus could decrease the fail and relapsing rate of 12 month,but could not increase the complete remission (P ＞ 0.05).(2) Comparing with cyclosporine A,tacrolimus had no difference in complete remission and the relapsing rate of 12 month (P ＞ 0.05),but could decrease the fail.(3) Tacrolimus could increase the complete remission and decrease fail,but had no difference in relapsing rate (P ＞ 0.05).(4) There was no significant difference in relapsing rate between tacrolimus and rituximab(P ＞ 0.05).(5) Tacrolimus had less side effects than cyclophosphamide.Conclusion Tacrolimus have advantages to cyclophosphamide,cyclosporine A and prednisolone,but not to rituximab,and have less side effects than cyclophosphamide.
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Objective To assess the efficacy of combined vitamin D with calcium and vitamin D or cal-cium alone in rickets of vitamin D deficiency by meta-analysis method. Methods Searches were made in Cochrance Library , Pubmed, Web of science, Scirus, CNKI, Chinese Biological Medical Literature Database, (CBM),Wangfang from the establishment of the data base till March 2013. All randomized controlled trials about combined with vitamin D and calcium in rickets of vitamin D deficiency were eligible. Serum 25-( OH) ritamin D,phosphate ,ALP,calcium,PTH,phosphate,albumin ,radiographic score were chosen as evaluation in-dex to evaluate the weighted mean diffreence ( WMD) and 95% confidence interval ( CI) for continuous data. RevMan 5. 0. 2 software was used to make meta-analysis. Results 437 literatures were reviewed. Three eligible trials were used for meta-analysis. Meta-analysis showed that:(1)the increase of Serum 25-(OH)vitamin D:Combined therapy group and vitamin D group (MD= -7. 88,95%CI:-12. 24~ -3. 52);Combined therapy group and calcium group (MD= -18. 32,95%CI:-22. 61~ -14. 04). (2)the increase of serum phosphate:Combined therapy group and vitamin D group ( MD= -0. 64 ( 95%CI:-0. 86 ~ -0. 42 );Combined therapy group and calcium group MD= -0. 16 ( 95%CI:-0. 84 ~0. 51 ) . ( 3 ) the decrease of Serum ALP:Combined therapy group and vitamin D group (MD= 109. 99,95%CI:20. 40 ~199. 58);Combined therapy group and calcium group (MD=59. 89,95%CI:10. 09~109. 59 ). (4)the increase of serum calcium :Combined therapy group and vitamin D group (MD= -0. 71,95%CI:-0. 91,-0. 52);the decrease of Radiographic score:Com-bined therapy group and vitamin D group( MD=0. 68,95%CI:0. 42~ 0. 95). Except that the increase of serum phosphate between combined therapy group and calcium group had no significant difference,the rest had signifi-cant difference. Conclusion The long term efficacy in combined therapy group is much effective than vitamin D group or calcium group.
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Objectives To investigate the effect of matrine (MAT) on adriamycin (ADR) induced podocyte injury in vitro and explore the function of the mammalian target of rapamycin (mTOR) protein signaling pathway during the intervention. Methods ADR (1μmol/L) was used to induce the model of podocyte injury and then the podocytes were intervened by 10, 20 and 40μg/ml MAT for 24 hours. The viability and apoptosis rate of podocytes, mRNA and protein expressions of desmin and mTOR were detected. The effect of different concentrations of MAT on ADR-induced podocytes was analyzed. Results Com-pared with the control group, declined viability of podocytes (P<0.001), increased percentage of apoptotic podocytes (P<0.001), and increased expression of damage marker desmin (P=0.002) were observed in ADR group. In ADR group, after intervention with MAT of 10-40μg/ml, increased viability of podocytes (P<0.05), decreased percentage of apoptotic podocytes (P<0.05), and decreased expression of damage marker Desmin were found (P<0.05). The protein expression of mTOR and phosphorylation state of mTOR (p-mTOR) decreased in ADR induced-podocytes (P<0.05), and after intervention with MAT of 10-40μg/ml, the expression of mTOR and p-mTOR increased (P<0.05). The expression of mTOR downstream target protein s6k1 and 4EBP1 mRNA decreased in ADR group (P=0.071), while increased after intervention with MAT of 10-40μg/ml (P<0.05). Conclusions 10-40μg/ml MAT have protective effects on ADR-induced podocytes in vitro. The protection mechanism may be related to mTOR signaling pathway.
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ObjectiveTo study the distribution characters and linkage disequilibrium of vitamin D receptor(VDR) gene ApaI and BsmI polymorphism,and explore the genetic susceptibility of VDR and vitamin D deficiency rickets.MethodsVDR gene ApaI and BsmI polymorphisms were determined by PCRRFLP technology in 56 cases of rickets and 76 cases of normal children.The frequencies of the VDR genotype and allele were compared between the two groups.The software SHEsis was used to make linkage disequilibrium analysis.Results No significant difference was found in either the frequency distribution of VDR gene ApaI and BsmI polymorphism or allele of them between two groups; Two polymorphisms didn't show linkage disequilibrium and D' and r2 were 0.23 and 0.01,respectively.ConclusionsThe ApaI and BsmI polymorphism of VDR gene might be not associated with vitamin D deficiency rickets.Two polymorphisms didn't show linkage disequilibrium.
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Objective To study the association of vitamin D receptor(VDR) gene BsmI polymorphism and the genetic susceptibility of vitamin D deficiency rickets in infants and to explore a new way of diagnosis and treat-ment. Methods Case-control study was adopted. 56 infants confirmed with rickets (case group) and 76 cases of normal infants (control group) were chosen as the subjects. PCR-RFLP was applied to examine VDR gene BsmI site polymorphism. The frequencies of the VDR genotype and allele were compared between the two groups. Results Frequencies of BB,Bb and bb genotypes were 3.6% (2/56),21.4% (12/56) and 75.0% (42/56) in the rickets group,and 1.3% (1/76),18.4% (14/76) and 80.3% (61/76) in the control group respectively(χ20.521,P> 0.05),frequencies of B,b alleles were 14.3% (16/112),85.7% (96/112) in the rickets group and 10.5% (16/152),89.5% (134/152) in the control group respectively(χ20.783,P>0.05). Multiple logistic regression analysis showed that VDR gene polymorphism Bsml had not higher risk of vitamin D deficiency rickets in Infants. Conclusion VDR gene polymorphism BsmI doesn't appear to pose risk on infants in developing vitamin D deficien-cy rickets.
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Objective To study the effect of oxymatrine on p-STAT3 and PIAS3 signaling molecule and it's mRNA expression in the proliferation of the human mesangial cells (HMCs) induced by lipopolysaccharide(LPS) and to explore their relationship. Methods HMCs were divided into three groups: control group, LPS group and oxymatrine group. HMC proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. Type Ⅳ collagen in the supernatants of the cultured HMCs was detected by ELISA at 12, 24, 48 hours respectively. At the same time, the protein and mRNA expressions of p-STAT3 and PIAS3 were measured by Western blot and real-time quantitative RT-PCR. Results The cell proliferation, the mRNA and protein expression of type Ⅳ collagen, p-STAT3 in LPS group were increased compared with the control group (P<0.01), but they were decreased in oxymatrine group (P < 0.01). The expressions of protein and mRNA of PIAS3 in LPS group were decreased significantly compared with control group (P<0.01), but they were increased in oxymatrine group (P<0.01). Conclusion Oxymatrine can down-regulate the expression of p-STAT3 and up-regulate the expression of PIAS3, which plays an important role in the process of LPS-induced HMCs proliferation.